Composite

Part:BBa_K2328055

Designed by: Yiyuan Gao   Group: iGEM17_TJU_China   (2017-10-20)


AcmA + Linker II + Linker.a

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 324
    Illegal NheI site found at 606
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 324
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Acma and smURFP are joined together, and the fragment is inserted into pMG36e to construct a new plasmid. When the plasmid is expressed, smURFP is anchored to the cell surface by Acma, thus expressing the shape. Linker II and Linker.a are used to separate Acma from other core parts .

Reference

[1] Li X, et al. Functional cell surface display of endo-beta-1, 3-1, 4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif.Sheng Wu Gong Cheng Xue Bao. 2009.

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